Reproductive and teratologic effects of low-frequency electromagnetic fields: A review of in vivo and in vitro studies using animal models

This systematic review evaluated the transplantation of cells derived from adipose tissue for applications in dentistry. SCOPUS, PUBMED and LILACS databases were searched for in vitro studies and pre-clinical animal model studies using the keywords “ADIPOSE”, “CELLS”, and “PERIODONTAL”, with the Boolean operator “AND”. A total of 160 titles and abstracts were identified, and 29 publications met the inclusion criteria, 14 in vitro and 15 in vivo studies. In vitro studies demonstrated that adipose- derived cells stimulate neovascularization, have osteogenic and odontogenic potential; besides adhesion, proliferation and differentiation on probable cell carriers. Preclinical studies described improvement of bone and periodontal healing with the association of adipose-derived cells and the carrier materials tested: Platelet Rich Plasma, Fibrin, Collagen and Synthetic polymer. There is evidence from the current in vitro and in vivo data indicating that adipose-derived cells may contribute to bone and periodontal regeneration. The small quantity of studies and the large variation on study designs, from animal models, cell sources and defect morphology, did not favor a meta-analysis. Additional studies need to be conducted to investigate the regeneration variability and the mechanisms of cell participation in the processes. An overview of animal models, cell sources, and scaffolds, as well as new perspectives are provided for future bone and periodontal regeneration study designs.

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Effects of pulsing electromagnetic fields on the prenatal and postnatal development in mice and rats: In vivo and in vitro studies

Teratology ◽

10.1002/tera.1420420207 ◽

1990 ◽

Vol 42 (2) ◽

pp. 157-170 ◽

Cited By ~ 38

Author(s):

Igor Zusman ◽

Perhija Yaffe ◽

Helena Pinus ◽

Asher Ornoy

Keyword(s):

Postnatal Development ◽

Electromagnetic Fields ◽

In Vitro Studies ◽

Prenatal And Postnatal Development

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Rabbits as Animal Models in Contemporary Implant Biomaterial Research

World Journal of Dentistry ◽

10.5005/jp-journals-10015-1069 ◽

2011 ◽

Vol 2 (2) ◽

pp. 129-134 ◽

Cited By ~ 1

Author(s):

Himanshu Arora ◽

Anil Nafria ◽

Anup Kanase

Keyword(s):

Animal Models ◽

Dental Implants ◽

Dental Implant ◽

Mechanical Stability ◽

In Vitro Studies ◽

Clinical Use ◽

Essential Step ◽

Rigorous Testing

ABSTRACT Development of an optimal interface between bone and orthopedic or dental implants has taken place for many years. In order to determine whether a newly developed implant material conforms to the requirements of biocompatibility, mechanical stability and safety, it must undergo rigorous testing both in vitro and in vivo. Results from in vitro studies can be difficult to extrapolate to the in vivo situation. For this reason the use of animal models is often an essential step in the testing of orthopedic and dental implants prior to clinical use in humans. This review discusses the reasons, the importance, and the research carried out in rabbits in our quest to develop a dental implant ideally suited for human bone.

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A REVIEW OF IN VITRO STUDIES: LOW-FREQUENCY ELECTROMAGNETIC FIELDS

AIHAJ ◽

10.1080/15298669391354531 ◽

1993 ◽

Vol 54 (4) ◽

pp. 178-185 ◽

Cited By ~ 44

Author(s):

Stephen F. Cleary

Keyword(s):

Electromagnetic Fields ◽

Low Frequency ◽

In Vitro Studies

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Evaluation of in vitro and in vivo anthelmintic efficacy of Cyperus compressus Linn., a traditionally used anthelmintic plant in parasite-animal models

Future Journal of Pharmaceutical Sciences ◽

10.1186/s43094-020-00148-5 ◽

2020 ◽

Vol 6 (1) ◽

Author(s):

Amar Deep Soren ◽

Arun Kumar Yadav

Keyword(s):

Animal Models ◽

Plant Extract ◽

In Vitro Studies ◽

In Vivo Studies ◽

Phytochemical Analysis ◽

Root Extract ◽

Reference Drug ◽

Anthelmintic Efficacy

Abstract Background The decoction of the roots of Cyperus compressus (Cyperaceae) is used to treat helminth infection by the Santhal tribe of Assam. The study evaluated the anthelmintic efficacy claims of the plant C. compressus through pre-clinical in vitro and in vivo studies employing available parasite-animal models such as Hymenolepis diminuta-Wistar rat (cestode) and Syphacia obvelata-Swiss mice (nematode) models. Results Phytochemical analysis revealed the presence of alkaloids, glycosides, reducing sugars, flavonoids, terpenoids, tannins, and steroids. In vitro studies were conducted employing H. diminuta and S. obvelata. In vitro studies against H. diminuta revealed mortality of parasites at 8.3 ± 0.05 h at the highest concentration of C. compressus methanolic root extract (30 mg/ml), whereas reference drug praziquantel (PZQ), showed mortality at 5.84 ± 0.01 h. Against S. obvelata, in the same concentration of the extract, mortality of parasites occurred in a much later time of 24.13 ± 0.03 h, whereas in the reference drug albendazole (ABZ), the parasites showed mortality at 7.24 ± 0.08 h. In vivo studies against H. diminuta revealed 61.74% reduction in the eggs per gram (EPG) counts and 24% reduction in worm counts at the highest dose of 700 mg/kg body weight (b.w.) of plant extract. Against S. obvelata, at 700 mg/kg b.w., 28.92% and 33.85% reduction in EPG and worm counts were recorded respectively. Conclusion Although the reference drugs showed better in vitro and in vivo efficacy, the plant extract showed a better in vitro efficacy against cestode parasite compared to its nematode counterpart indicating that it possesses a better cestocidal efficacy. EPG reductions were higher against H. diminuta, whereas worm count reduction was higher against S. obvelata. The findings justify the use of C. compressus as an anthelmintic in the traditional medicine of the Santhals of India.

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Abnormal Sensitivity of Cortisol-Producing Adrenocortical Adenomas to Serotonin: In Vivo and in Vitro Studies

Yearbook of Endocrinology ◽

10.1016/s0084-3741(08)70169-0 ◽

2007 ◽

Vol 2007 ◽

pp. 358-361

Author(s):

D.E. Schteingart

Keyword(s):

In Vitro Studies ◽

Adrenocortical Adenomas

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The Venom of the Boomslang (Dispholidus Typus): In Vivo and in Vitro Studies

Thrombosis and Haemostasis ◽

10.1055/s-0038-1653532 ◽

1969 ◽

Vol 21 (02) ◽

pp. 234-244 ◽

Cited By ~ 5

Author(s):

N Mackay ◽

J.C Ferguson ◽

Antonia Bagshawe ◽

A.T.T Forrester ◽

G.P Mcnicol

Keyword(s):

In Vitro Studies

SummaryAn account is given of the effects of boomslang venom in man. Evidence was found of a fibrinolytic state apparently secondary to the coagulant action of the venom. These features rapidly responded to the administration of specific antivenom. In vitro studies, using a homogenate of boomslang parotids, confirmed the coagulant properties of the venom and showed them to be of much greater potency than the proteolytic actions.

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